期刊简介

       本刊是由黑龙江省教育厅主管、佳木斯大学主办的高等医学院校学报,属医药学学术理论刊物,适于医学研究生、本科生及广大医务工作者临床科研,其主要宗旨在于报道科研学术动态,传播医药学学术信息,促进医药学的发展。                

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  • 杂志名称:黑龙江医药科学杂志
  • 主管单位:黑龙江省教育厅
  • 主办单位:佳木斯大学
  • 国际刊号:1008-0104
  • 国内刊号:23-1421/R
  • 出版周期:双月刊
期刊荣誉:黑龙江省优秀期刊期刊收录:维普收录(中), 万方收录(中), 国家图书馆馆藏, 知网收录(中), 上海图书馆馆藏
黑龙江医药科学杂志2003年第05期

关键词:neural stem cells, IGF-1, Bcl-2, protection
摘要:Objective: To study the effect of IGF-1 on the expression of Bcl-2 of NSCs and the protective effect of Bcl-2 on the NSCs deprived of browth factors. Methods: The neural stem cells wereisolated from hippocampi of newborn rats and were cultured by using serum-free medium containing EGF 20ng/ml and bFGF 20ng/ml. Expression of Bcl-2 and nestin antigen were observed by using immunocytochemistry method, MTT method was used to analyse the vitality change of NSCs cultured with IGF-1 and without IGF-1 after withdrawing growth factors. Results: After cultured 7d with IGF-1,the cells were almost positive expression of nestin antigen, and (21.3±0.4)% of all NSCs were Bcl-2 positive cells, furthermore, these cells had a larger survival term against withdrawing growth factors than that cultured without IGF-1.Conclusion: IGF-1 could promote the expression of Bcl-2 in NSCs without affecting the characters of positive expression of nestin antigen, moreover, could increase the self-protection ability through elevating expression of Bcl-2 against deprivation of growth factors in the NSCs.